Progress in Stem Cell <p>Progress in Stem Cell (PSC) (ISSN 2199-4633) is the best Open Access journal that acts as a forum for translational research into stem cell therapies. PSC is scientific journal that overlays the study of Cancer stem cells, Stem cell therapy, Stem-cell transplantation, human embryonic stem cells, Neural stem cells, Murine Embryonic Stem Cells, Adult stem cell, Pancreatic stem cells, etc. PSC is a peer-reviewed journal that focuses on the areas of established and emerging concepts in stem cell research and their assorted disease therapies. It provides an opportunity to share the scientific information among the clinical &amp; medical scientists and researchers.</p> Biomedpress en-US Progress in Stem Cell 2199-4633 <p>Copyright The Author(s) 2017. This article is published with open access by <a href="" target="_blank">BioMedPress</a>. This article is distributed under the terms of the&nbsp;<a href="" target="_blank">Creative Commons Attribution License (CC-BY 4.0)</a> which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.&nbsp;</p> L-ascorbic acid-2-phosphate and Fibrin Substrate Enhances the Chondrogenic Differentiation of Human Adipose-derived Stem Cells <p><strong>Background</strong>: Adipose-derived stem cells (ASCs) have been developed as a substitute for chondrocytes in cartilage tissue engineering. This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human ASCs cultured on fibrin substrate.</p> <p><strong>Methods</strong>: Human ASCs were isolated by an enzymatic process and characterized. The fibrin scaffold was fabricated from human blood as a natural scaffold. The ASCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 mg/ml) and a control without an ascorbic acid supplementation. The chondrogenic differentiation of the ASCs was evaluated involving glycosaminoglycan analysis, and the mRNA expression of type 2 collagen, aggrecan and type 1 collagen.</p> <p><strong>Results</strong>: Chondrogenic differentiation occurred when the ASCs were supplemented by 3 mg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan. The expression of type 1 collagen, which is an osteogenic marker, was low.</p> <p><strong>Conclusions</strong>: ASCs with a supplementation of 3mg/ml of ascorbic acid cultured on a fibrin substrate promotes chondrogenic differentiation.</p> Imam Rosadi Karina Karina Viol Dhea Kharisma Arif Nur Muhammad Ansori ##submission.copyrightStatement## 2021-12-31 2021-12-31 8 2 310 317 10.15419/psc.v8i2.412 title description none g