Progress in Stem Cell 2020-01-19T08:57:39+00:00 Lili Hami Open Journal Systems Therapeutic role of bone marrow-derived stem cells and zinc sulfate for reduction of liver fibrosis 2020-01-19T08:57:39+00:00 Arsheema Kazmi Abeer Kazmi Sulaiman Shams Abdul Sajid Khalid Khan <p><strong>Introduction</strong>: Hepatic fibrosis is considered as the initial state of any chronic hepatic disease; progression causes accumulation of extracellular matrix (ECM) and develops into cirrhosis. In case of liver failure, transplantation was considered as the only suitable therapy but due to the hurdles in transplantation, therapy using stem cells was promoted. Mesenchymal stem cells (MSCs) are widely used for the treatment of a variety of diseases. Oxidative stress at the damage site causes poor MSC proliferation and engraftment. Thus, it is necessary to induce antioxidants with MSCs to enhance potency.</p> <p><strong>Methods</strong>: To explore the therapeutic potential of zinc sulfate (ZnSO<sub>4</sub>) and MSCs on carbon tetra chloride (CCl<sub>4</sub>)-induced hepatic toxicity, 6-8 week-old female albino mice (<em>BALB/c</em>) were used. Initially, mice were infected with CCl<sub>4</sub> and then intra-peritoneally injected with MSCs only, ZnSO<sub>4</sub> only, or a combination of MSCs and ZnSO<sub>4</sub>. MSCs were isolated from femur and tibia of mice, and were cultured under control conditions.</p> <p><strong>Results</strong>: The morphological results revealed that in contrast to MSC-only therapy, ZnSO<sub>4</sub> enhanced the therapeutic potential of MSCs when administered to CCl<sub>4</sub>-injured mice. Biochemically, level of serum Alanine Transaminase (ALT) and total bilirubin was found to be significantly decreased in ZnSO<sub>4</sub> + MSC transplanted mice. Histopathological examination also revealed that ZnSO<sub>4</sub> + MSC transplantation induced a strong anti-apoptotic effect on CCl<sub>4</sub>-injured liver. Reverse transcription polymerase chain reaction (RT-PCR) detected a noteworthy anti-fibrotic effect of MSCs in the presence of ZnSO<sub>4</sub>, down-regulation of apoptotic marker (<em>Bax</em>), and up-regulation of anti-apoptotic (<em>Bcl-xl</em>) and hepatic (<em>Albumin</em>) markers.</p> <p><strong>Conclusion</strong>: Thus, it was concluded that the presence of ZnSO<sub>4</sub> reduced oxidative stress, enhanced the proliferation rate of MSCs, and significantly attenuated hepatic fibrosis.</p> <p>&nbsp;</p> 2019-12-21T00:00:00+00:00 ##submission.copyrightStatement##