Progress in Stem Cell 2019-03-21T05:51:47+00:00 Lili Hami Open Journal Systems An evolution of stem cell research and therapy in Viet Nam 2019-03-21T05:51:47+00:00 Phuc Van Pham Ngoc Bich Vu Oanh Thuy Huynh Mai Thi-Hoang Truong Truc Le-Buu Pham Long Thanh Dang Ngoc Kim Phan Kiet Dinh Truong <p>Stem cell research is among the most exciting, attractive, and rapidly growing fields in biomedicine. Besides the popularity of stem cell research at the bench, stem cell therapy has become a bustling interest in the clinic and industry. In Vietnam, biomedical scientists have studied stem cells since 1995. From that time on, Vietnamese scientists have obtained significant achievements in stem cell research and therapy, especially with regards to stem cell therapy for disease treatment. This report aims to provide an overview of stem cell research and therapy from 1995 to date. Stem cell research activities were collected by questionnaire and analyzed based on publications and projects about stem cells in various databases, including Pubmed, Web of Science, Google Scholar, Embase, as well as from national scientific information. The results showed that stem cell research and therapy significantly increased from 2009 to date with greater publications on stem cells and clinical applications. With this growth rate, Vietnam is poised to continue developing in the stem cell industry. The potential goal for Vietnam is to advance as one of the countries in the Southeast Asian region and in the world that continues to push the frontier in stem cell research and therapy.</p> 2018-01-30T00:00:00+00:00 ##submission.copyrightStatement## Expression of CD34 marker in the adipose tissue derived stem cells 2019-03-21T05:51:44+00:00 Phuc Van Pham Ngoc Bich Vu Van Hong Tran <p><strong>Introduction</strong>: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marker of hematopoietic stem cells. This study aimed to analyze and determine the expression of CD34 marker in ten samples of ADSCs obtained from 10 donors.</p> <p><strong>Methods</strong>: All ADSC samples were isolated and expanded according to the published previous protocols. They were confirmed as the MSCs with some markers and differentiation potential, excepting the CD34 expression. Then they were cultured and analyzed the expression of CD34 by flow cytometry at passage 3, 5, 7 and 9.</p> <p><strong>Results:</strong> The results showed that expression of CD34 in ADSCs was different between donors and their passages that accounted from 1.21% to 23.38%.</p> <p><strong>Conclusion</strong>: These results suggested that ADSCs are not ‘truly” MSCs like MSCs from bone marrow.</p> 2018-03-01T00:00:00+00:00 ##submission.copyrightStatement##